Volume 8, Number 1, March 2018
|Number of page(s)||6|
|Published online||26 February 2018|
Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Iranian Center of Excellence in Health Management, Tabriz University of Medical Sciences, Tabriz, Iran
3 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, I.R. Iran
4 Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, I.R. Iran
5 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, I.R. Iran
6 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, I.R. Iran
* Corresponding authors. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. E-mail addresses: Aghazadehm@tbzmed.ac.ir (M.- Aghazadeh), Kafilhs@tbzmed.ac.ir (H. S. Kafil).
Accepted: 12 December 2017
Background: The presence of Class 1, 2 and 3 integrons in clinical isolates of Pseudomonas aeruginosa with multi-drug resistance phenotype has rendered the organism as a new concern. Objective: This study aimed to investigate the prevalence of Class 1, 2 and 3 integrons in multi-drug resistant clinical isolates of Pseudomonas aeruginosa collected from hospitals in the city of Tabriz
Materials and Methods: A total of 200 P. aeruginosa non-duplicated clinical isolates were collected from inpatients and outpatients in different wards of hospitals from May to November 2016. The bacteria were identified by conventional microbiological methods. Antibiotic susceptibility test was performed by disk diffusion method and the presence of integrons was analyzed by polymerase chain reaction (PCR).
Results: Colistin was the most effective antibiotic, while 98% of the isolates were resistant to cefotaxime. Fifty-three percent of the isolates were recorded as multi-drug resistant (MDR) phenotype; however, 27.5% of the isolates were resistant to more than 8 antibiotics. In this study, 55 (27.5%), 51 (25.5%), and 30 (15%) clinical isolates of P. aeruginosa were positive for Class 1, 2 and 3 integrons, respectively. aac(6)II in Class I integrons and dfrA1 in ClassII and aacA7 in Class II integrons were the most prevalent genes. Resistance to aminoglycosides were the most common genes harbored by integrons.
Conclusion: The results of this study showed that the prevalence of Class 1, 2 and 3 in integron genes in most P. aeruginosa strains islated from different parts and equipment used in the hospital. The role of these transferable genetic agents has been proven in the creation of resistance. Therefore, it is essential to use management practices to optimize the use of antibiotics, preferably based on the results of antibiogram and trace coding genes for antibiotic resistance.
Key words: Pseudomonas aeruginosa / Antibiotic resistance / Integrons / Iran
© Author(s) 2018. This article is published with open access by China Medical University
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