Volume 9, Number 2, June 2019
|Number of page(s)||11|
|Published online||24 May 2019|
Protective effect of Glycyrrhiza glabra roots extract on bone mineral density of ovariectomized rats
Laboratory for Research of the Musculoskeletal System (LRMS), School of Medicine, National and Kapodistrian University of Athens, KAT Hospital, Athens, Greece
2 1st Department of Orthopaedics, National and Kapodistrian University of Athens, Faculty of Medicine, Attiko Hospital, Athens, Greece
3 Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Greece
4 Hellenic Red Cross Hospital, Athens, Greece
5 Lab. of Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki School of Veterinary Medicine,
6 Department of Mechanics, National Technical University of Athens (NTUA), National Technical University of Athens, Greece
* Corresponding author. National and Kapodistrian University of Athens Athens, Greece. E-mail address: firstname.lastname@example.org (D. Galanis)
Accepted: 9 March 2019
Objective: The aim of this study was to evaluate the potential effect of the methanolic extract of plant Glycyrrhiza glabra roots on bone mineral density and femoral bone strength of ovariectomized rats.
Methods: Thirty 10-month-old Wistar rats were randomly separated into three groups of ten, Control, Ovariectomy and Ovariectomy-plus-Glycyrrhiza in their drinking water. Total and proximal tibial bone mineral density was measured in all groups before ovariectomy (baseline) and after 3 and 6 months post ovariectomy. Three-point-bending of the femurs and uterine weight and histology were examined at the end of the study.
Results: No significant difference was noted in bone density percentage change of total tibia from baseline to 3 months between Control and Ovariectomy-plus-Glycyrrhiza groups (+5.31% ± 4.75 and +3.30% ± 6.31 respectively, P = non significant), and of proximal tibia accordingly (+5.58% ± 6.92 and +2.61% ± 13.62, P = non significant) demonstrating a strong osteoprotective effect. There was notable difference in percentage change of total tibia from baseline to 6 months between groups Ovariectomy and Ovariectomy-plus-Glycyrrhiza (−13.03% ± 5.11 and −0.84% ± 7.63 respectively, P < 0.005), and of proximal tibia accordingly (−27.9% ± 3.69 and −0.81% ± 14.85 respectively, P < 0.001), confirming the protective effect of Glycyrrhiza glabra extract in preserving bone density of the Ovariectomy-plus-Glycyrrhiza group. Three-point-bending did not reveal any statistically significant difference between Ovariectomy and Ovariectomy-plus-Glycyrrhiza groups. Uterine weights of the Ovariectomy-plus-Glycyrrhiza group ranged between the other two groups with no statistically significant difference to each.
Conclusions: Glycyrrhiza glabra root extract notably protected tibial bone mineral density loss in Ovariectomy-plus-Glycyrrhiza rats in comparison with ovariectomized rats, but did not improve biomechanical strength.
Key words: Rat / Osteoporosis / Ovariectomy / Absorptiometry / Three-point-bending
© Author(s) 2019. This article is published with open access by China Medical University
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