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Fig. 2

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KLEXJ extract promotes ALP activity and BMP-2 expression through the PI3K pathway. (A) Osteoblasts were pretreated with Ly294002 (10 μM) for 30 min or transfected with p85 siRNA for 24 h, followed by stimulation with KLEXJ for 48 h; ALP activity was examined with a commercial ALP assay kit. (B&C) Osteoblasts were pretreated with Ly294002 (10 μM) for 30 min or transfected with p85 siRNA for 24 h, followed by stimulation with KLEXJ for 24 h; BMP-2 expression was examined by qPCR and Western blot analysis. (D) Osteoblasts were incubated with KLEXJ for indicated time intervals and p85 phosphorylation was examined by Western blot analysis. Results are expressed as mean ± S.E.M.*, p < 0.05 compared with control. #, p < 0 .05 compared with KLEXJ-treated group.