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Effects of YC-1 on cell viability and cell confluence in CAR cells. Cells were incubated with 0, 25, 50 and 100 μM of YC-1 for various duration. (A) The cell viability was determined by MTT assay. (B) The cell confluence was determined by the IncuCyte™ Kinetic Live Cell Imaging System. Data are presented as the mean ± sd (n = 3). ***p < 0.001 versus untreated control.