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Effects of YC-1 on ROS, mitochondrial membrane potential (∆Ψm) and the levels of apoptosis-related proteins in CAR cells. Cells were incubated with 0, 25, 50, 75 and 100 of YC-1 for 48 h. (A) ROS level was assessed by staining with H2DCFDA, and (B) loss of A¥m was measured with DiOC6(3) by flow cytometry. Data are presented as the mean ± sd (n = 3). ***P < 0.001 versus untreated control. (C) Whole-cell lysates were prepared, and the levels of apoptosis related proteins were analyzed by western blot analysis Data is presented.