Prevalence of integrons 1, 2, 3 associated with antibiotic resistance in Pseudomonas aeruginosa isolates from Northwest of Iran

Shahram Mobaraki; Mohammad Aghazadeh; Mohammad Hossein Soroush Barhaghi; Mohammad Yousef Memar; Hamid Reza Goli; Pourya Gholizadeh; Hossein Samadi Kafil

doi:10.1051/bmdcn/2018080102

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Volume 8 / No 1 (March 2018)

BioMedicine, 8 1 (2018) 2

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Table 1

List of primers were used for the PCR amplification and sequencing of integrons in the present study.

Target region	Primer sequence (5́ → 3́)	Size of product	Annealing Temperature	References
intI-1F	TCATGGCTTGTTATGACTGT GTAGGGCTTATTATGCACGC	600 bp^*	57̊C	26
intI-1R
intI-2F	GATGCCATCGCAAGTACGAG CGGGATCCCGGACGGCATGCACGATTTGTA	750 bp^*	57̊C	27
intI-2R
intI-3F	GCCTCCGGCAGCGACTTTCAG ACGGATCTGCCAAACCTGACT	650 bp^*	59̊C	28
intI-3R

^*

bp = base pair, F = forward sequence, R = reverse sequence.

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