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Effects of BITC on cell confluence of CAR cells. Cells were incubated with 0, 5, 10, or 20 μM of BITC for various durations. (A) Cell morphology and (B) cell confluence were determined by an IncuCyte ZOOM System instrument. Data are presented as the means ± SD (n = 3). ***p < 0.001 versus untreated control.