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Effects of BITC on DAPI staining and TUNEL assay on CAR cells. Cells were harvested following treatment with or without 10 μM of BITC for 48 h, and 4’-6-diamidino-2-phenylindole (DAPI) solution (1 μg/ml) and an In Situ Cell Death Detection Kit, Fluorescein were used. (A) The image of TUNEL positive cells (green fluorescence) were shown in CAR cells after 10 μM BITC treatment for 48 h. (B) Apoptotic cells were qualified as described in the Materials and Methods section. The data are shown as the means ± SD (n = 3). ***p<0.05 compared to untreated cells.