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Effects of the pan-caspase inhibitor Z-VAD-fmk on apoptotic death in BITC-treated CAR cells. Cells were pretreated in the presence or absence of 10 μM Z-VAD-fmk and then exposed to 10 μM BITC for 48 h. (A) Cell morphologic observation was photographed. (B) Cell viability was assessed by MTT assay. The data are shown as the means ± SD (n = 3). ***p<0.05 compared to BITC-treated cells.