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Effects of BITC on ROS production and mitochondrial membrane potential (Δψm) in CAR cells. Cells were incubated with 0, 2.5, 5, and 10 μM of BITC for 48 h. (A) ROS level was assessed by staining with H2DCFDA, and (B) loss of Δψm was measured with DiOC(3)6 by flow cytometry. Data are presented as the means ± SD (n = 3). ***p < 0.001 versus untreated control.