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Fig. 7

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Effects of BITC on apoptotic signaling of CAR cells. Cells were treated without or with 2.5, 5, and 10 μM of BITC for 48 h, and cell lysates were collected and blotted using specific antibodies, including Bax, Bad, Bcl-2, Bcl-xL, cytochrome c, caspase-9 and caspase-3 by immunoblotting analysis as described in the Materials and Methods section. Each lane of protein signaling was normalized to β-actin.